Select the best alignment according to different measure of sequence conservation


The table describes for each sequence alignment basic statistics and the top oligonucleotides considering the following measures of sequence conservation:
-PIS: percentage of identical sites
-3'PIS: percentage of identical sites in the last five nucleotides at the 3' end of oligonucleotide
-PPI: percentage of pairwise identity


Summary of conservation values per alignment

Conservation measure EbolaID_alig01 EbolaID_alig02 EbolaID_alig03 EbolaID_alig04 EbolaID_alig05 EbolaID_alig06 EbolaID_alig07
Global Statistics Sequences
Length
% Identical sites
% Pairwise identity
99
22790
20.8
71.8
101
18960
90.9
98.9
124
19560
34.8
85.7
160
24236
16
85.5
160
24236
16
85.5
215
20233
37
92.4
1611
19241
5.6
98.6
% of identical sites Average
Range
Top Primer or Probe
27.90
0-60
EBOLAID042
EBOLAID051
94.64
70-100
EBOLAID001
EBOLAID002
EBOLAID004
55.29
0-94.74
EBOLAID039

41.46
0-84.21
EBOLAID039

41.46
0-84.21
EBOLAID039

57.95
5-89.47
EBOLAID039

31.41
0-90
EBOLAID054

3' end % of identical sites Average
Range
Top Primer or Probe
30.00
0-100
EBOLAID057

95.25
80-100
EBOLAID001
EBOLAID002
EBOLAID003
60.00
0-100
EBOLAID017
EBOLAID023
EBOLAID025
48.00
0-100
EBOLAID017
EBOLAID027
EBOLAID036
48.00
0-100
EBOLAID017
EBOLAID027
EBOLAID036
62.25
0-100
EBOLAID023
EBOLAID025
EBOLAID027
57.75
0-100
EBOLAID004
EBOLAID005
EBOLAID006
% of pairwise identity Average
Range
Top Primer or Probe
75.55
57.59-91.4
EBOLAID042

99.28
94.86-100
EBOLAID001
EBOLAID002
EBOLAID004
91.03
73.31-98.3
EBOLAID039

91.71
81.46-99
EBOLAID040

91.71
81.46-99
EBOLAID040

95.59
88.07-99.02
EBOLAID039

99.91
99.17-100
EBOLAID030
EBOLAID042
EBOLAID046